Validating internal controls for quantitative plant gene expression studies

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We investigated the ability of RNA interference (RNAi) directed against two co-orthologs of AGAMOUS (AG) from Malus domestica (domestic apple, Md AG) to reduce the risks of invasiveness and provide genetic containment of transgenes, while also promoting the attractiveness of flowers for ornamental usage.

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Quantitative reverse transcription – polymerase chain reaction (q RT-PCR) has been demonstrated to be particularly suitable for the analysis of weakly expressed genes, such as those encoding transcription factors.

However, accurate measurement of gene expression with this method relies on the choice of a valid reference for data normalization.

Studies rarely verify that gene expression levels for reference genes are adequately consistent among the...

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validating internal controls for quantitative plant gene expression studies-16

The key lessons from our experience are: (1) stable gene expression is the rule in vegetatively propagated transgenic poplars; (2) somaclonal variation is modest and...We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising.For further information, including about cookie settings, please read our Cookie Policy .Rice (Oryza sativa L.) is an important crop and the most advanced model for monocotyledonous species; its nuclear genome has been sequenced and molecular tools are being developed for functional analyses.However, high-throughput methods for rice research are still limited and a large-scale q RT-PCR platform for gene expression analyses has not been reported.

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