Validating internal controls for quantitative plant gene expression studies
We investigated the ability of RNA interference (RNAi) directed against two co-orthologs of AGAMOUS (AG) from Malus domestica (domestic apple, Md AG) to reduce the risks of invasiveness and provide genetic containment of transgenes, while also promoting the attractiveness of flowers for ornamental usage.
The cookies contain no personally identifiable information and have no effect once you leave the Medscape site.
Quantitative reverse transcription – polymerase chain reaction (q RT-PCR) has been demonstrated to be particularly suitable for the analysis of weakly expressed genes, such as those encoding transcription factors.
However, accurate measurement of gene expression with this method relies on the choice of a valid reference for data normalization.
Studies rarely verify that gene expression levels for reference genes are adequately consistent among the...